基于RNA-Seq筛选鸭肠炎病毒感染鸭脾差异表达免疫相关基因

doi:10.11843/j.issn.0366-6964.2017.02.013

Abstract

Abstract:

The aim of the present study was to screen the differentially expressed immune-related genes from duck spleen by DEV infection. Ducks of 50 days old were selected to inoculate with DEV in leg muscle, and the duck spleens were collected at 66, 90 and 114 h after inoculation, and were extracted for total RNA, then sequencing by high-throughput RNA-Seq technology. The sequenced segments were compared and noted for screening of the differentially expressed immune-related genes by GO and KEGG database in NCBI. Some of the differentially expressed genes were selected to verify by real-time PCR technology. The results showed that there were 511 of the differentially expressed genes in duck spleen at 66 h after DEV infection, which 70 involved in the biological immune process, including 46 up-regulated genes and 24 down-regulated genes. At 90 h after DEV infection, there were 485 of the differentially expressed genes, which 64 involved in the biological immune process, including 49 up-regulated genes and 15 down-regulated genes. At 114 h after DEV infection, there were 531 of the differentially expressed genes, which 66 involved in the biological immune process, including 35 up-regulated genes and 31 down-regulated genes. The differentially expressed immune-related genes were mainly involved in cell adhesion, antigen processing and presentation and complement activation through GO database analysis. And these genes were mainly enriched in the signaling pathways as cell adhesion molecule, ECM receptor interaction and PPAR, etc. The expressed contents of the selected 7 genes by the FQ-PCR, were basically the same with the sequencing results by RNA-Seq technology. These results provided a theoretical basis for further understanding of the mechanism of DEV infection and duck immune respone.

CLC Number:

WU Liang-tao, ZHENG Min, HUA Min, WAN Run, CHENG Zhen-tao, ZHOU Bi-jun, WEN Ming. Screening of Differentially Expressed Immune-related Genes from Duck Spleen with Duck Enteritis Virus Infection Based on RNA-Seq Technology[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 297-306.

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Screening of Differentially Expressed Immune-related Genes from Duck Spleen with Duck Enteritis Virus Infection Based on RNA-Seq Technology

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